Abstract
Isolated bacterial cytoplasmic membrane vesicles have provided a useful model system for studies of active transport (KABACK, 1970a, b; KABACK 1972; HONG, KABACK, 1973; KABACK, 1973; KABACK, in press). Vesicles are devoid of the cytoplasmic constituents of the intact cell, and their metabolic activities are restricted to those provided by the enzymes of the membrane itself. This constitutes a considerable advantage over intact cells in the study of certain transport mechanisms, since active transport by membrane vesicles is practically nil in the absence of the appropriate exogenous energy source. Thus, the energy source for transport of a particular substrate can be determined by studying which substances stimulate accumulation. Moreover, metabolic conversion of the transport substrate and the energy source is minimal, allowing clear definition of the reactions involved.
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