Abstract
A study on isolation and antioxidant activity of secondary metabolit of methanol extract of fruit of Senduduk Bulu (Clidemia hirta (L.) D. Don) has been carried out. Senduduk bulu is used for folk medicine to many diseases. Flavonoid and phenolic has been reported to its activity as antioxidant. The aim of this research was to find antioxidant compound and antioxidant activity of this fruit. Isolation works were conducted using Vacuum Liquid Chromatography and Column Chromatography method. Antioxidant activity was determined using DPPH free radical scavenging methods. The isolation works afforded 10 mg of pure compound CHP1 which was obtained as white crystal, with its melting point of 165–167ºC, soluble in chloroform and slightly soluble in methanol. This compound gave positive reaction with Liebermann-Burchard (LB) reagent and concluded as terpenoid. Based on it UV spectrum, this compound showed a λ max of 316 nm. Its FT-IR spectrum showed appearence of some functional groups of OH, C-H aliphatic, C=O ester,C=C alkene, CH bending and C-O. The 1H-NMR spectrum showed the alkene protons and methyl protons which is a characteristic of terpenoid compounds. The pure compound (CHP1) provided a weak antioxidant activity with the IC50 of 327,01µg/mL.
Highlights
A study on isolation and antioxidant activity of secondary metabolit of methanol extract of fruit of Senduduk Bulu (Clidemia hirta (L.) D
Senduduk bulu is used for folk medicine to many diseases
Flavonoid and phenolic has been reported to its activity as antioxidant. The aim
Summary
Melastomataceae diantaranya adalah senyawa fenolik, flavonoid, tannin, terpenoid, saponin, steroid, glikosida, dan alkaloid. Diketahui beberapa spesies dari famili ini memiliki beberapa aktivitas seperti antioksidan, antihipertensi, antihiperglikemik, homeo-statis, antihepatitis dan antidiare (Tjitraresmi et al, 2018). Beberapa spesies Melastomataceae yang diketahui mengandung senyawa fenolik yang cukup besar yaitu Melastoma malabathricum sebesar 384,33 mg AG/g, pada Meriania hernandoi sebesar 240 mg AG/g dan pada. IC50 sebesar 7,14 μg/mL dan ekstrak etanol daun Clidemia hirta berpotensi sebagai antiproliferasi terhadap sel kanker Dalton’s. Lymphoma Ascites (DLA) dengan nilai IC50 sebesar 68 μg/mL (Tjitraresmi et al, 2018)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
