Abstract
The fungus-inoculated leaflets of 55 Trifolium species have been examined for the presence of isoflavonoid and non-flavonoid phytoalexins. Isoflavonoid derivatives belonging to the pterocarpan (medicarpin, maackiain and 4-methoxymaackiain) and isoflavan (vestitol, isovestitol, sativan, isosativan and arvensan) classes were isolated from 50 species whilst T. campestre and T. dubium accumulated the trihydroxy stilbene, transresveratrol. Three other species ( T. badium, T. scutatum and T. spadiceum) apparently did not produce phytoalexins in response to fungal inoculation. The distribution and biosynthetic relationship of Trifolium phytoalexins is discussed and the genus compared with others belonging to the Trifolieae and related tribes.
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