Abstract
In this study, we investigated the effect of isochoric moisture heating on the functionalities of simply fractionated soy protein. Soy protein-rich fractions (SPFs) were produced through a simplified wet fractionation process, in which the defatting and intensive washing steps were omitted. After a neutralization step, the protein-rich dispersions were heated up to various temperatures ranging from 60 to 100 °C and subsequently freeze-dried. It was found that the denaturation level, the water holding capacity and the viscosity of SPFs increased as the processing temperature rose, whereas the solubility of SPFs decreased. Additionally, among the different conditions, an intermediate heating temperature of 70 °C resulted in SPFs having a gel network with the highest elasticity. Overall, isochoric moisture heating coupled to simplified fractionation can be used to precisely control the functionality of soy protein ingredients with mild heating conditions. This work provides insights on the production of ingredients with desired functionality for novel soy-based applications like soy meat analogues.
Highlights
Diets containing more plant protein are stimulated due to reasons of environmental concerns, vegetarianism trends and increased consumer acceptance of plant-based products (Pojic, Misan, & Tiwari, 2018)
The protein and oil content of all the Soy protein-rich fractions (SPFs) were quite similar since the isochoric moisture heating step was carried out after all the separation steps before freeze-drying (Table 1)
The product yield was an average of 27% while the protein yield reached up to 56%. These results were in line with the yields reported by our previous research
Summary
Diets containing more plant protein are stimulated due to reasons of environmental concerns, vegetarianism trends and increased consumer acceptance of plant-based products (Pojic, Misan, & Tiwari, 2018). These changed diet habits lead to a higher demand for plant-based ingredients with the proper functionality to make new products. There is a growing interest to develop simplified wet fractionation to avoid the use of organic solvents (especially during the oil extraction step) and intensive washing steps (Berghout, Boom, & Van Der Goot, 2014; Campbell et al, 2011; Peng, Dewi, Kyriakopoulou, & van der Goot, 2020). Simplified fractionation can be rated as applicable, effective and potentially as a more sustainable alternative enabling solvent-free label status (Geerts, van Veghel, Zisopoulos, van der ; Nazir, Asghar, & Aslam Maan, 2016)
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