Abstract
Cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance to Verticillium wilt. This results in serious losses to both yield and fiber quality. To identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the central Arabidopsis thaliana defense regulator Enhanced Disease Susceptibility 1 (EDS1). The full-length cDNA of GbEDS1 was obtained by screening the full-length cDNA library of Gossypium barbadense combining with RACE strategy. Its open reading frame is 1848 bp long, encoding 615 amino acid residues. Sequence analysis showed that GbEDS1 contains a conserved N-terminal lipase domain and an EDS1-specific KNEDT motif. Expression profiling indicated that the gene is induced by Verticillium dahliae as well as salicylic acid (SA) treatment. Subcellular localization assays revealed that GbEDS1 is located in the cell cytoplasm and nucleus. Overexpression of GbEDS1 in Arabidopsis dramatically up-regulated SA and H2O2 production, resulting in enhanced disease resistance to V. dahliae. Silencing of GbEDS1 in G. barbadense significantly decreased SA and H2O2 accumulation, leading to the cotton more susceptibility. Moreover, combining the gene expression results from transgenic Arabidopsis and silenced-GbEDS1 cotton, it indicated that GbEDS1 could activate GbNDR1 and GbBAK1 expression. These findings not only broaden our knowledge about the biological role of GbEDS1, but also provide new insights into the defense mechanisms of GbEDS1 against V. dahliae in cotton.
Highlights
Cotton (Gossypium sp.) is one of the most economically valuable crops globally
In that putative lipase domain, the C-terminal region contains the specific motif KNEDT, which is highly conserved among EDS1 sequences from Populus poplar (Pp), Vitis vinifera (Vv), and A. thaliana (At) (Figure 1; Table 1), suggesting that our Gossypium barbadense enhanced disease susceptibility 1 (GbEDS1) is an ortholog of AtEDS1 and VvEDS1
The results suggested that GbEDS1 played a crucial role in Verticillium wilt resistance responses by regulating the accumulation of salicylic acid (SA), H2O2
Summary
Cotton (Gossypium sp.) is one of the most economically valuable crops globally. Because its products are used as textile fibers, feed stock, foodstuff, oil, and biofuels. Verticillium wilt, induced by Verticillium dahliae Kleb., is one of the most destructive diseases of G. hirsutum L., and has been detected in most areas where that species is cultivated. Losses to annual yields have risen to more than 1.5 million bales worldwide (Cai et al, 2009). This soil-borne pathogen penetrates the roots and systemically infects the plant through the xylem (Daayf et al, 1995; Klosterman et al, 2009). The introduction of genetic resistance is considered the best and most sustainable management approach, a lack of information about the molecular mechanisms for cotton resistance against V. dahliae has slowed progress in those research efforts
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