Abstract
Today, vitrification of human blastocysts provides an excellent outcome although a big water filled cavity called the blastocoele needs to be dehydrated prior to the cooling steps of vitrification. The replacement of water by the cryoprotectant seems to be the supported by artificially collapsing of the blastocoel. Therefore we started to apply artificial collapsing on human blastocysts using a laser pulse prior to vitrification, and then compared outcome data with frozen embryo transfers (FET), where no artificial collapse was applied.
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