Abstract
Traditional fixed-time evaluation (FTE) of fertilization at 18 hours after fertilization and embryo morphology on day 3 could miss information on fertilization status if the pronuclei appear (PNa) after 18 hours or pronuclei fade (PNf) before 18 hours after fertilization, as well as information on embryo development before day 3. This study examined the developmental potential of embryos with PNa greater than 18 hours or PNf less than 18 hours after fertilization, and of embryos with irregular division (ID) or with multinucleated blastomeres (MN) during days 1 and 2 using time lapse monitoring (TLM).
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