Abstract
Objectives: The use of round spermatids for human fertilization has met with limited success perhaps because of the immaturity of the spermatids, the difficulty in their identification and the poor rates of oocyte activation with these cells. The objectives of this study were to: 1) develop an animal model for round spermatid injection (ROSI) using non-human primate oocytes; 2) to examine the cytoskeletal events after rhesus ROSI and 3) to perform ICSI ‘rescue’ on failed ROSI fertilizations with subsequent embryo development and transfer.
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