Abstract
Neuroblastomas are solid tumors and mostly seen in the adrenal medulla and sympathetic ganglia. It is known that neuroblastoma cell proliferation is inhibited by cisplatin and vincristine. The aim of this study was to investigate the effect of oxytocin on cell viability in human neuroblastoma SH-SY5Y cell line and primary cerebral cortex cell culture exposed to cisplatin and vincristine. In this direction, SH-SY5Y cell line and cortex neurons were obtained from the medical pharmacology department, Ataturk University. Both cells were grown in the appropriate cell culture media. Cisplatin (5, 10, 15 μg), vincristine (0.5, 1 and 2 ng) and oxytocin (1 μM) were applied to SH-SY5Y cell line and primary cortex cell culture for 24 h. MTT and TAC-TOS tests were performed 24 h after the application. As a result of the MTT assay, the combination of cisplatin and vincristine reduced cell viability in both cultures approximately 25% and 22%, respectively, compared to the control group. It appears that oxytocin increases neuroblastoma and cortex neuron viability, 112% and 95%, respectively. In this relation, we need to investigate why oxytocin increases cell viability and what are the possible implications in women in lactation stage.
Highlights
Neuroblastoma (NB), derived from neural cells, is a tumor which is seen in the adrenal medulla and sympathetic ganglia
MTT test: To determine cell viability, the MTT assay test was used after 24 h exposure to cisplatin, vincristine and oxytocin combination with cisplatin and vincristine (Figure 1)
Oxytocin combination with cisplatin and vincristine in neuron cells in all doses did not show any significant difference in comparison with the control group
Summary
Neuroblastoma (NB), derived from neural cells, is a tumor which is seen in the adrenal medulla and sympathetic ganglia. NB is the most common extracranial solid tumor of early childhood neoplasms, which accounts for 15% of all childhood cancer deaths. Despite all current advancements in drug delivery systems and cancer therapy, neuroblastoma displays an aggressive, therapyresistant phenotype, and prognosis remains poor [1]. Most chemotherapeutic agents used in the clinic for cancer therapy induce cell cycle arrest and apoptosis. Cisplatin (CP) is an inorganic platinum complex, which is mostly effective against many tumors. Cytotoxicity of cisplatin is based on cisplatin-derived DNA inserts, including protein-DNA cross-links, DNA mono adducts, and interstrand or intrastrand cross-links and the formation of reactive oxygen species (ROS) in cells [2]
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