Is genetic polymorphism of ER-α, CYP1A1, and CYP1B1 a risk factor for uterine leiomyoma?

Abstract

Uterine leiomyoma is the most common benign smooth muscle tumor. This study was carried out to evaluate the association of ER-α, CYP1A1, and CYP1B1 polymorphisms with uterine leiomyoma in Egyptian women. The study population consisted of 160 patients with uterine leiomyoma and 100 healthy women as control. The genetic polymorphisms for ER-α MSP1 exon 1, CYP1B1 Leu432Val, and CYP1A1 Ile462Val were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing methods. There were no statistically significant differences in the overall associations between the ER-α exon I CT genotypes and uterine leiomyoma (P = 0.47). However, an elevated risk of uterine leiomyoma was observed among women with the CYP1A1 Ile462Val AG genotype (P = 0.07) and CYP1B1 Leu 432Val C/C genotype (P = 0.08). We concluded that the carriage of CYP1A1 Ile462Val AG and CYP1B1 Leu 432Val CC genotypes predict the susceptibility to leiomyoma in Egyptian women and they are likely to contribute in the pathogenesis of leiomyoma.