Abstract
BackgroundMalignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin. Recent studies have suggested that long non-coding RNAs (lncRNAs) play crucial regulatory roles in multiple malignancies, including melanoma. Testis expressed 41 (TEX41) is a relatively new lncRNA whose mechanism in melanoma remains vague.AimsThis study aimed to explore the role and specific mechanism of TEX41 in melanoma.MethodsThe expression of genes involved in this study was determined by qRT-PCR. Functional assays were conducted to analyze the role of relevant genes in melanoma cells. The interaction between TEX41 promoter and IRF4 as well as the relationship among TEX41, miR-103a-3p and C1QB was verified by mechanism assays.ResultsIRF4 up-regulated TEX41 at the transcriptional level in melanoma cells. TEX41 knockdown hindered melanoma cell proliferation, migration and invasion while promoting cell apoptosis. TEX41 bound to miR-103a-3p and regulated C1QB. The suppressive impact of TEX41 depletion on melanoma cell malignant behaviors could be counteracted by miR-103a-3p inhibition or C1QB overexpression. Moreover, IRF4 could facilitate melanoma cell growth via up-regulating C1QB.ConclusionsIRF4-activated TEX41 sequestered miR-103a-3p and modulated C1QB to promote melanoma cell malignant behaviors, for which TEX41 might be regarded as a potential therapeutic target for melanoma.
Highlights
Malignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin
The suppressive impact of Testis expressed 41 (TEX41) depletion on melanoma cell malignant behaviors could be counteracted by miR-103a-3p inhibition or Complement C1q B chain (C1QB) overexpression
Interferon regulatory factor4 (IRF4)-activated TEX41 sequestered miR-103a-3p and modulated C1QB to promote melanoma cell malignant behaviors, for which TEX41 might be regarded as a potential therapeutic target for melanoma
Summary
Malignant melanoma is an aggressive skin cancer and a tumor of melanocytic origin. Recent studies have suggested that long non-coding RNAs (lncRNAs) play crucial regulatory roles in multiple malignancies, including melanoma. Testis expressed 41 (TEX41) is a relatively new lncRNA whose mechanism in melanoma remains vague. Malignant melanoma, derived from melanocytes [1], is a type of aggressive cancer that occurs on the body surface or in internal organs [2]. It Multiple studies have shown that the abnormal expression of long non-coding RNAs (lncRNAs) is involved in the occurrence and development of tumors and diseases, including melanoma [5,6,7]. LncRNA TEX41 is a new research target involved in a variety of cancers by sponging different miRNAs
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