Abstract
The Ca 2+ ionophore A23187 (0.2–5 μM) stimulates the phosphorylation of the substrates of protein kinase C (40,000 dalton protein) and myosin light chain kinase (20,000 dalton protein) in the presence or absence of cyclooxygenase inhibitors. In the presence of cyclooxygenase inhibitors or millimolar Ca 2+ there is no stimulation of phospholipase C by A23187. Fingerprints of the 32P-labeled 40,000 dalton protein isolated from platelets that have been stimulated with A23187, thrombin, phorbol 12, 13-dibutyrate and 1,2-didecanoylglycerol were identical. Higher concentrations of A23187 (1–5 μM) induced the loss of polyphosphoinositides through phosphomonoesterase activity.
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