Abstract
In this work, surface disinfection and biofilm susceptibility were investigated by applying ionic silver of 0.4–1.6 µg/mL and cathodic voltage-controlled electrical treatment of 1.8 V and a current of 30 mA to Escherichia coli (E. coli) ATCC 25922 biofilm-contaminated titanium substrates. Herein, it is evident that the treatment exhibited the potential use to enhance the susceptibility of bacterial biofilms for surface disinfection. In vitro studies have demonstrated that the ionic silver treatment of 60 min significantly increased the logarithmic reduction (LR) of bacterial populations on disinfectant-treated substrates and the electrical treatment enhanced the silver susceptibility of E. coli biofilms. The LR values after the ionic silver treatments and the electric-enhanced silver treatments were in the ranges of 1.94–2.25 and 2.10–2.73, respectively. The treatment was also associated with morphological changes in silver-treated E. coli cells and biofilm-contaminated titanium surfaces. Nevertheless, the treatments showed no cytotoxic effects on the L929 mouse skin fibroblast cell line and only a slight decrease in pH was observed during the electrical polarization of titanium substrate.
Highlights
Biofilms are communities of bacteria that form when cells attach to a surface and secrete a matrix of extracellular polymeric substance (EPS)
The surface disinfection and biofilm susceptibility were investigated by applying ionic silver of 0.4–1.6 μg/mL and cathodic voltage-controlled electrical treatment of 1.8 V and a current of 30 mA, for 60 min to E. coli ATCC 25922 biofilm on titanium substrate as a pathogenic Gram-negative bacteria model
The results revealed that the electrical treatment effectively eTnahbalenc1e. dDethtaeilssilovfeerxspuesricmepetnitbaillittryeaotfmEe.nctosl.i biofilms
Summary
Biofilms are communities of bacteria that form when cells attach to a surface and secrete a matrix of extracellular polymeric substance (EPS). Killing microorganisms in biofilm can require 500 to 5000 times the concentrations of antimicrobial agents effective on their planktonic counterparts [1,2]. Pathogenic bacterial colonization of dental implants and the infection of peri-implant tissues can lead to chronic bone destruction and may lead to implant failure [3]. Peri-implantitis is an infectious disease of bacterial origin [4] that causes an inflammatory process in soft tissues around an osseointegrated dental implant in function and results in loss of the supporting bone [5]. The traditional treatment for peri-implantitis includes mechanical decontamination and local antiseptic or antibiotic treatment [6,7]. Suggested dental implant surface treatments are scaling, CO2 lasers, air abrasive powder, chlorhexidine or hydrogen peroxide irrigation, or local antibiotics. Most of the information on the effectiveness of such interventions derives from case reports, so that no evidence-based consensus has been reached as to which option is clinically most advantageous
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