Inwardly Rectifying K+Channels in Dispersed Bovine Parathyroid Cells

Abstract

Excitation-secretion coupling in various endocrine cells is dependent on membrane voltage which is controlled by ion channels. In order to characterize and determine the functional significance of voltage-gated ion channels in the parathyroid cell, the patch clamp technique was used in cell-attached and whole cell configurations to study single channel and whole cell currents in dispersed bovine parathyroid cells. Whole cell voltage clamp recordings from dissociated bovine parathyroid cells were obtained in a physiologic solution containing (in mM): 140 NaCl, 5.4 KCl, 2 CaCl2, and 2 MgCl2. The pipette (intracellular) solution contained (in mM) 145 KAsp, 10−5CaCl2, and 2 MgCl2. Currents were recorded in response to 20-mV incremental changes in voltage of 300-ms duration every 3 s from −80 to +40 mV and from −40 to −140 mV. There was a small outward current recorded in response to 300-ms pulses of 20-mV increments from −80 to +40 mV. A large inward current was recorded following hyperpolarization of the parathyroid cell from −40 to −140 mV. The reversal potential for the current was −60 to −65 mV, suggesting that the majority of the current is carried by a channel that is K+selective. Our results suggest that the whole cell currents of dispersed bovine parathyroid cells in physiologic extracellular solution include an in inwardly rectifying K+current which is open at low intracellular calcium concentration. This inwardly rectifying K+channel is likely to play a major role in maintaining negative membrane potential by opposing calcium-induced depolarization of the parathyroid cell and, as a result, may have an important role in regulation of PTH secretion.