Abstract
A convenient method for determination of enamel solution rate in vivo has been developed. The two upper central incisors are etched simultaneously for one or two minutes by a packet containing cellophane film moistened with 1.5 m lactate buffer at pH 4.5. Calcium diffusing into the cellophane is transferred in vitro to a second cellophane film containing alizarin sulphonate and the calcium alizarin sulphonate formed is freed of excess reagent by a timed rinse. The cellophane is cemented to glass and the stable precipitate in the film is read densitometrically. Conditions of the test have been studied to optimize calcium fixation and rinse conditions, to verify stability of absorbance, to determine linearity and sensitivity, and to study the variables associated with the etch solution, the etch duration and the enamel surface conditions. The sensitivity of the method has been maximized in order to minimize the risk to experimental subjects.
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