Abstract
A half century ago, Green Fluorescent Protein (GFP) from a pacific jellyfish was isolated as byproduct in the purification of the chemiluminescent protein Aeqourin. While the latter was later turned into a calcium-sensitive probe, GFP was treated like a fluorescent curiosity in biophysical research for almost three decades. Once it was recognized that fluorescence appears without the need of cofactors, the transfection into other organisms proved the autocatalytical character of chromophore formation and boosted the research in life sciences. The discovery of the GFP, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins, was consequently awarded with the Nobel Prize for Chemistry in 2008.In my presentation, I will review the highlights in the history of GFP and other fluorescent proteins from a spectroscopist's point of view. Furthermore, open questions and new ideas how fluorescent proteins can be turned into biosensors will be discussed.
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