Abstract
We present a method for localised two-photon photoconversion of fluorescent cells within distinct microanatomical compartments in intact lymph nodes. This method allows efficient photoconversion of whole cell populations as well as of single cells while preserving cell viability and motility. Moreover, the combination of high-speed image acquisition and real-time display permits us to target and lock-on to individual cells for photoconversion as they migrate within the lymph node. Optical cell marking makes it possible to differentiate otherwise homogenous responding cells based on their microanatomical location. It also enables discontinuous in vivo tracking of cell fates to distal sites after extended time intervals.
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