Investigations on electromigration phenomena for protein crystallization using crystal growth cells with multiple electrodes: effect of the potential control

Abstract

Investigations on electromigration phenomena for protein crystallization in gels and solution using a crystal growth cell with multiple electrodes at constant current (2 μA per circuit) are discussed. In this work, the advantages and disadvantages of different electrode dispositions number, and electrode connections inside the cell are shown. A pH change effect was discarded as the main factor affecting the selective growth of the crystals at the cathode and the fact is explained in terms of electromigration phenomena. Additionally, controlled potential experiments were carried out in order to avoid parasitic reactions observed at constant current, resulting in an increase of reproducibility and better control of crystal growth.