Investigations by time-resolved laser-induced fluorescence and capillary electrophoresis of the uranyl–phosphate species: application to blood serum

Abstract

An understanding of the speciation of uranium(VI) (uranyl ion, UO 2 2+) in blood medium is very important for investigations of internal contamination. Studies have been performed on the speciation of uranium(VI) in the presence of phosphate and blood serum using time-resolved laser-induced fluorescence (TRLIF) and capillary electrophoresis (CE). By TRLIF it is possible to identify spectrally and temporally, by comparison with distribution curves calculated using data from the OECD database, UO 2H 2PO 4 +, UO 2HPO 4 and UO 2PO 4 − species. Results concerning the study of the uranium(VI)–blood plasma system are presented. CE has demonstrated that it is possible to separate human γ-, β-, α 2-, and α 1-globulins and albumin in the presence of uranium(VI).