Abstract
In the present study, the investigation includes the estrus activity after synchronization of estrus by using 20 mg impregnated sponges with Medroxy Progesterone Acetate for 12 days with 400 IU I/M injection of pregnant mare serum gonadotropin 24 hrs. before sponges withdrawal. All 30 Iraqi sheep's showed (100%) estrus after sponge withdrawal. Twin percentage 40% while single percentage 60%. The polymorphisms of gonadotropin-releasing hormone receptor gene were analyzed as a genetic marker in 30 Iraqi sheep's. Two noticed genotypes (GA and GT), and single nucleotide polymorphisms in the exon2 region of gonadotropin-releasing hormone receptor gene as genetic marker and correlated with high litter size after hormonal super ovulation response in sheep. The frequencies of alleles G,A and G,T in Iraqi ovine breeds were 0.50 and 0.50, respectively the outcomes revealed that GA, GT genotype was related with better litter-size in Iraqi ovine breeds. Therefore, these outcomes recommend that gonadotropin-releasing hormone receptor gene is a powerful candidate gene that impacts litter-size in sheep.
Highlights
I/M injection of pregnant mare serum gonadotropin 24 hrs. before sponges withdrawal
Sequence of nucleotides of exon 2 of gonadotropinreleasing hormone (GnRHR) gene by using BioEdit program, which was performed by National Instrumentation Center for Environmental Management (NICEM) online at
The same outcomes was described (14 and 15) when the pregnant mare serum gonadotropin (PMSG) hormone given 24 hrs. before the removal of sponge, the does come in heat during the 1236 hrs. by removing the sponge
Summary
All 30 Iraqi sheep's showed (100%) estrus after sponge withdrawal. The polymorphisms of gonadotropin-releasing hormone receptor gene were analyzed as a genetic marker in 30 Iraqi sheep. There were two noticed genotypes (GA and GT), and single nucleotide polymorphisms in the exon region of gonadotropin-releasing hormone receptor gene as genetic marker and correlated with high litter size after hormonal super ovulation response in sheep. The frequencies of alleles G,A and G,T in Iraqi ovine breeds were 0.50 and 0.50, respectively. The result revealed that GA, GT genotype was related with better litter-size in Iraqi ovine breeds. These outcomes recommend that gonadotropin-releasing hormone receptor gene be a powerful candidate gene that impacts litter-size in sheep
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