Abstract
A new metabolite of chloramphenicol (CAP) has been isolated from incubation mixtures containing CAP and liver microsomes from phenobarbital-pretreated rats. This compound has been identified as the oxamic acid derivative of CAP by reverse isotope dilution, high pressure liquid chromatography, and isobutane chemical ionization mass spectroscopy. It has also been identified as a product of the chemical hydrolysis of radiolabeled microsomal protein. These results support the proposal that CAP is metabolically activated by liver microsomes to an oxamyl chloride reactive intermediate which either hydrolyzes to the oxamic acid or acylates protein.
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