Abstract

Fully methyl-esterified, water-soluble citrus pectin was partially hydrolysed with alkali to different degrees of esterification (DE). High-field 13C-NMR spectroscopy of the products showed that ester carbonyl carbons appeared as four signals due to different influences from neigh-bouring sugar residues (free or methyl-esterified galactosyluronic acid). Based on the relative peak areas of these signals, it was deduced that alkaline de-esterification occurred randomly. The distribution of methyl ester groups was also found to be random in water-soluble native pectins of tobacco (DE 20) and papaya (DE 70).

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