Investigation of platelet membrane glycoprotein Ibα gene polymorphisms in children with Kawasaki disease

Abstract

Objective To investigate the association between platelet glycoprotein Ibα (GPIbα) gene HPA-2a/b polymorphisms and the risk of Kawasaki disease (KD) and that complicated with coronary artery lesion (CAL). Methods A total of 30 patients with KD and 60 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis for the HPA-2a/b polymorphism in GPIbα gene. Results For HPA-2a/b polymorphism in GPIbα gene, there was only genotype TC and CC of HPA-2a/b polymorphism in GPIbα gene in children with KD and healthy controls, and genotype TT was not found in both groups.There were no significant differences between KD patients and the controls in genotype frequencies of CC, TC and TT and allele frequencies of C and T (χ2=0.052, 0.048, all P>0.05); also there was no significant difference between KD patients with CAL and that without CAL in genotype and allele frequencies (χ2=2.672, 2.481, all P>0.05). Conclusion No association is found between HPA-2a/b polymorphism in GPIbα gene and the risk of KD or its complication of CAL in this study. Key words: Platelet glycoprotein Ibα; Kawasaki disease; Gene polymorphism