Abstract
Plasma metabolomics and lipidomics have been commonly used for biomarker discovery. Studies in white and Japanese populations suggested that gender and age can affect circulating plasma metabolite profiles; however, the metabolomics characteristics in Chinese population has not been surveyed. In our study, we applied liquid chromatography-mass spectrometry-based approach to analyze Chinese plasma metabolome and lipidome in a cohort of 534 healthy adults (aging from 15 to 79). Fatty-acid metabolism was found to be gender- and age-dependent in Chinese, similar with metabolomics characteristics in Japanese and white populations. Differently, lipids, such as TGs and DGs, were found to be gender-independent in Chinese population. Moreover, nicotinate and nicotinamide metabolism was found to be specifically age-related in Chinese. The application of plasma metabolome and lipidome for renal cell carcinoma diagnosis (143 RCC patients and 34 benign kidney tumor patients) showed good accuracy, with an area under the curve (AUC) of 0.971 for distinction from healthy control, and 0.839 for distinction from the benign. Bile acid metabolism was found to be related to RCC probably combination with intestinal microflora. Definition of the variation and characteristics of Chinese normal plasma metabolome and lipidome might provide a basis for disease biomarker analysis.
Highlights
Plasma has been widely used for biomarker exploration in various diseases
Our study provides an overview of plasma metabolomic characteristics of the Chinese population and a new insight into Renal cell carcinoma (RCC) diagnosis
Determination of the normal inter-individual variations range of metabolites in healthy population would provide baseline reference for future biomarker discovery
Summary
Plasma has been widely used for biomarker exploration in various diseases. It is a “data-rich” source that contains several thousands of metabolites and would likely reflect the contributions from various organs. Plasma metabolites are more stable due to regulation via human homeostasis [1]. Lipidomics is a component of plasma metabolomics, which comprehensively analyse the lipid metabolites in the plasma. The definition of the characteristics of the normal plasma metabolome and lipidome would provide a basis for disease analysis, as well as for the understanding of a healthy plasma metabolism state.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
