Investigation of Phonon-Like Excitations in Hydrated Protein Powders by Neutron Scattering

Abstract

Detecting the phonon dispersion relations in proteins is essential for understanding the intra-protein dynamical behavior. Such study has been attempted by X-ray in recent years [1,2]. However, for such detections, neutrons have significant advantages in resolution and time-efficiency compare to X-rays. Traditionally the collective motions of atoms in protein molecules are hard to detect using neutrons, because of high incoherent scattering background from intrinsic hydrogen atoms in the protein molecules. The recent availability of a fully deuterated green fluorescent protein (GFP) synthesized by the Bio-Deuteration Lab at ORNL opens new possibilities to probe collective excitations in proteins using inelastic neutron scattering [3]. Using a direct time-of-flight Fermi chopper neutron spectrometer, we obtained a full map of the milli-eV phonon-like excitations in the fully deuterated protein. The Q range of the observed excitations corresponds to the length scale of about 2.5 to 3 Å, which is close to the length scales of the secondary structures of proteins (4-5 Å) and reflects the collective intra-protein motions. Our results show that hydration of GFP seems to harden, not soften, the collective motions, which is counterintuitive, but in agreement with the observations by previous neutron scattering experiments [4]. [1] D. Liu, X.-Q. Chu, M. Lagi, Y. Zhang, E. Fratini, P. Baglioni, A. Alatas, A. Said, E. Alp, and S.-H. Chen, Phys. Rev. Lett. 101, 135501 (2008). [2] M. Li, X.-Q. Chu, E. Fratini, P. Baglioni, A. Alatas, E. Alp, and S.-H. Chen, Soft Matter 7, 9848-9853 (2011). [3] X.-Q. Chu, U. Shrestha, H. O'Neill, Q. Zhang, A. I. Kolesnikov, and E. Mamontov, "Mapping meV excitations in a fully deuterated green fluorescent protein by time-of-flight neutron scattering", to be submitted. [4] X.-Q. Chu, et. al, J. Phys. Chem. B 113, 5001 (2009).