Abstract
Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which affect the gastrointestinal tract with intermittent ulceration. It is increasingly clear that neutrophil extracellular traps (NETs) seem to have a role in IBD; however, the associated pathogenesis is still not known. Furthermore, several conventional therapies are available against IBD, although these might have side effects. Our current study aimed to investigate the effects of hydrogen sulfide (H2S) treatment on NETs formation and on the expression of inflammatory mediators in experimental rat colitis. To model IBD, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was administered intracolonically (i.c.) to Wistar–Harlan male rats. Animals were treated (2 times/day) with H2S donor Lawesson’s reagent per os. Our results showed that H2S treatment significantly decreased the extent of colonic lesions. Furthermore, the expression of members of NETs formation: peptidyl arginine deiminase 4 (PAD4), citrullinated histone H3 (citH3), myeloperoxidase (MPO) and inflammatory regulators, such as nuclear transcription factor-kappa B (NF-κB) and high-mobility group box 1 (HMGB1) were reduced in H2S treated group compared to TNBS. Additionally, H2S donor administration elevated the expression of ubiquitin C-terminal hydroxylase L1 (UCHL-1), a potential anti-inflammatory mediator. Taken together, our results showed that H2S may exert anti-inflammatory effect through the inhibition of NETs formation, which suggests a new therapeutic approach against IBD.
Highlights
Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which affect the gastrointestinal (GI) tract with intermittent ulceration
Significantly enhanced expression of ubiquitin C-terminal hydroxylase L1 (UCHL-1) the H2 Sindonor group compared to trinitrobenzenesulfonic acid (TNBS) (0.42 ± 0.06 vs. 0.18 ± 0.03 relative expression)
Similar to Lawesson’s reagent, we observed a significant increase in the positive control (SASP) group compared to TNBS
Summary
Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which affect the gastrointestinal (GI) tract with intermittent ulceration. Crohn’s disease (CD) and ulcerative colitis (UC) are the two major types of IBD with similar symptoms but different epidemiological and clinical features [2]. Both disorders are characterized by phases of relapses and remissions, weight loss, fever, abdominal pain, diarrhea, and rectal bleeding [3]. These symptoms affect the quality of life of the patients, so the primary purposes of IBD therapy are the induction and maintenance of remission along with symptomatic treatments [4]. The development of new therapies and potential therapeutic targets are urgently needed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
