Abstract
BackgroundNatural products derived from medicinal plants are a major source of drug preparation and the main basis for the development of pharmaceutical leads. We have aimed at investigating in vitro antibacterial and antioxidant activity of various extracts of Citrus medica L. against a number of human pathogenic bacteria.MethodsThe plant samples of C. medica L were collected from Ramsar province, Iran. The gram-positive bacteria Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Enterococcus faecalis, and Staphylococcus aureus, as well as the gram-negative bacteria Escherichia coli, Shigella boydii, Salmonella typhi, Pseudomonas aeruginosa, Enterobacter aerogenes and Klebsiella pneumoniae were prepared from Bu Ali Sina University, Hamadan, Iran. Agar diffusion assay was applied, and the antioxidant properties of extracts were determined by DPPH assay. Total phenolic and flavonoid contents as well as some compounds such as alkaloids, saponin, and tannin were further analyzed.ResultsResults indicated that C. medica extracts possessed antibacterial activity, and that root, seed, and leaf exhibited the highest activities against human pathogens, especially M. luteus. Roots contained the highest total phenolics (106.1 mgGA/g), while leaves contained the highest total flavonoids (3.24 mgQ/g). Leaf methanol extract also contained alkaloids, saponins, and tannins.ConclusionsThe antibacterial activities of C. medica extracts could be explained by synthesizing such compounds. Moreover, seed and root extracts of C. medica showed strong radical scavenging activities
Highlights
Natural products derived from medicinal plants are a major source of drug preparation and the main basis for the development of pharmaceutical leads
Antibacterial Activity The inhibitory effects of different alcoholic and aqueous extracts of root, seed, and leaf of Citrus medica were evaluated against human pathogenic bacteria (Table 1)
The inhibitory activity of root methanol extract on M. luteus and E. aerogenes and that of root ethanol extract on E. aerogenes was more potent than gentamicin
Summary
Natural products derived from medicinal plants are a major source of drug preparation and the main basis for the development of pharmaceutical leads. We have aimed at investigating in vitro antibacterial and antioxidant activity of various extracts of Citrus medica L. against a number of human pathogenic bacteria. Total phenolic and flavonoid contents as well as some compounds such as alkaloids, saponin, and tannin were further analyzed. Results: Results indicated that C. medica extracts possessed antibacterial activity, and that root, seed, and leaf exhibited the highest activities against human pathogens, especially M. luteus. Citrus medica L. is a valuable medicinal plant used in Iranian ethnomedicine. It is a small plant, having short thorns and large and rectangular leaves with elliptical fruits. The root extract of C. medica is used for its anthelmintic and antilithic properties in the treatment of urinary calculi in India [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Avicenna Journal of Clinical Microbiology and Infection
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
