Abstract
BackgroundOral cavity cancer ranks the sixth most common malignancy worldwide, of which oral squamous cell carcinoma is the predominant type. This study aimed to investigate the function and the underlying mechanism of resistin like beta (RETNLB) in oral squamous cell carcinoma.MethodsThe data of oral squamous cell carcinoma samples from The Cancer Genome Atlas database was used to examine RETNLB expression and assess its correlation with the clinical outcomes. Biological functions of RETNLB on the growth, invasion and migration of cells were determined by cell counting kit 8, clonogenic growth, and Transwell assays. Gene set enrichment analysis was utilized to identify the important gene sets associated with RETNLB expression, which was further confirmed by western blot.ResultsWe found that RETNLB was upregulated in oral squamous cell carcinoma tissues and cells. High expression of RETNLB was closely linked to age and pathological tumor, and significantly related to poor survival of oral squamous cell carcinoma patients. Further functional experiments showed that knockdown of RETNLB significantly reduced the viability, mobility and invasiveness of cells. Moreover, gene set enrichment analysis suggested that Toll-like receptor signaling pathway was significantly correlated with high RETNLB expression. Further western blot analysis verified that silencing RETNLB could notably suppress the protein levels of Toll-like receptor 2, Toll-like receptor 4 and phosphor- extracellular signal-regulated kinase.ConclusionsThese results suggested that downregulation of RETNLB may restrain the progression of oral squamous cell carcinoma by inactivating TLR/2/4/ERK pathway.
Highlights
Oral cavity cancer is a part of head and neck cancer, which ranks the sixth most common malignancy worldwide [1]
resistin like beta (RETNLB) is upregulated in oral squamous cell carcinoma and involved in poor outcomes To illuminate the expression of RETNLB in oral squamous cell carcinoma, the levels of RETNLB were firstly examined in 338 oral squamous cell carcinoma tissues and 32 adjacent non-tumor tissues based on the The Cancer Genome Atlas database
RETNLB was observed to be overexpressed in two commonly used oral squamous cell carcinoma cell lines in comparison with non-tumor cell line (p < 0.01, Fig. 1b), suggesting that RETNLB might play a carcinogenic effect in oral squamous cell carcinoma
Summary
Oral cavity cancer is a part of head and neck cancer, which ranks the sixth most common malignancy worldwide [1]. Oral squamous cell carcinoma is the predominant type of oral cavity cancer, accounting for 90% [3]. Functional experiments demonstrated that RETNLB-overexpression could remarkably enhance the invasiveness and mobility of gastric carcinoma cells and promote the progression of epithelial-mesenchymal transition [16]. These evidences suggest that RETNLB is expected to be a potential biomarker in some tumors, and led us to suspect that RETNLB may play a role in oral squamous cell carcinoma, which has not yet been reported. Oral cavity cancer ranks the sixth most common malignancy worldwide, of which oral squamous cell carcinoma is the predominant type. This study aimed to investigate the function and the underlying mechanism of resistin like beta (RETNLB) in oral squamous cell carcinoma
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