Abstract
Invertase (EC 3.2.1.26) is an important enzyme in carbohydrate metabolism. This sucrose hydrolyzing enzyme was extracted and partially purified from mature flowers stored at -75° C, and fresh flower buds of snapdragon (Anthirrinum majus L.). Purification steps were precipitation with ammonium sulfate (25% to 80%) and two column chromatography fractionations: DEAE-Sephacel followed by Sephacryl S-200. An acid invertase was found in flowers of `Maryland White' and also in flower buds of `Potomac White'. Maximum enzyme activity was obtained at 55° C and a pH range from 4.2 to 5.0 for both enzymes in flowers and flower buds. Compared to citrate, acetate, citrate phosphate, and succinate buffers at pH 4.6 and 0.1 M increased enzyme activity by two fold.
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