Abstract

The invertase activity ( v) and ethanol production rate ( E) were measured during the fed-batch culture of yeast on sugar cane blackstrap molasses under different feeding rates, which were calculated by equation: F = F 0.e − Kt (the fermentor filling-up-time was equal to 7 h). The highest total invertase activity (41900 U) and ethanol production rate (76.6 g/h) were obtained when the mash was added into the reactor following a exponentially decreasing feeding rate at K = 0.6 h −1 ( F 0 = 4.3 l h ).

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