Abstract
The measurement of metabolite concentrations in animal tissues has become an important branch of enzymatic analysis. The measurements of changes in metabolite patterns in tissues in response to the alterations of substrate or hormonal balance have provided considerable information on the regulation of metabolic pathways. This chapter discusses the method to determine the concentrations of metabolites in animal tissues. It presents the tables that are meant to be a guide to the metabolite concentrations likely to occur in vivo under a variety of different conditions; only values that have been obtained with an enzymatic method on tissue and have been freeze-clamped or immersed in a refrigerant are included. In the chapter, the values are expressed in μmole/g fresh weight of tissue or μmole/ml whole blood except where otherwise stated. An important advance in the study of brain metabolites is the development of an apparatus that removes and freezes the brain of the conscious rat within a second, by blowing it out of the cranial vault into a thin layer between two metal disks cooled in liquid nitrogen.
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