Abstract
Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. Bone marrow transplantation (BMT) has been widely performed in patients with blood disorders and cancers. However, the cellular-level behaviors of the transplanted bone marrow cells over wide-areas of the host bone marrow after the BMT are not fully understood yet. In this work, we performed a longitudinal wide-area cellular-level observation of the calvarial bone marrow after the BMT in vivo. Using a H2B-GFP/β-actin-DsRed double-transgenic mouse model as a donor, a subcellular-level nuclear-cytoplasmic visualization of the transplanted bone marrow cells was achieved, which enabled a direct in vivo dynamic monitoring of the distribution and proliferation of the transplanted bone marrow cells. The same spots in the wide-area of the calvarial bone marrow were repeatedly identified using fluorescently labeled vasculature as a distinct landmark. It revealed various dynamic cellular-level behaviors of the transplanted BM cells in early stage such as cluster formation, migration, and active proliferation in vivo.
Highlights
Bone marrow (BM) inside the bone is a soft flexible tissue that produces the majority of erythrocytes, thrombocytes, and immune cells
Using a custom-built laser-scanning confocal platform combined with a stereotaxic mount (S1 Fig), we performed an intravital imaging of transplanted bone marrow cells at the calvarial bone marrow (BM) of the recipient mouse
From day 0 to day 1 after the bone marrow transplantation (BMT), the transplanted BM cells observed in the calvarial bone marrow were greatly increased in number and sparsely distributed
Summary
Bone marrow (BM) inside the bone is a soft flexible tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. Approximately 200 billion blood cells are newly produced in the human bone marrow. As the source of this massive cellular production, bone marrow houses hematopoietic stem cells (HSCs) that can differentiate into various kinds of blood cells such as erythrocytes, thrombocytes, and immune cells including neutrophils and lymphocytes [1,2,3]. Due to its critical role in the homeostasis of the circulatory and immune cellular systems, HSC has been widely used in various clinical situations, mainly in the form of bone marrow transplantation (BMT) [4, 5]. BMT has been performed in patients with blood.
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