Abstract
Triggering the CD3/TCR complex of lymphocytes induces a rapid rise in cytosolic free calcium followed by a slowly declining plateau. The level of this plateau depends on external pH, the more alkalinized media leading to higher values. Neither a pH-dependent binding of mAb, nor a pertubation of internal pH can account for this effect. In a sodium-free medium, or in the presence of dimethylamiloride Ca 2+, elevation is accompanied by an acidification of the cells, both of them depend, to the same extent, on external calcium concentration. TPA inhibits CD3-, but not ionomycin-induced Ca 2+ and H + raises, indicating that it acts more probably on Ca 2+ influx, rather than on its efflux. These results suggest that intracellular calcium could be regulated by a Ca 2+/H + ATPase which drives H + in and Ca 2+ out. In the presence of external Na +, H + should return to the medium by the Na +/H + exchanger.
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