Interaction of neuronal nitric oxide synthase with alpha1-adrenergic receptor subtypes in transfected HEK-293 cells

Abstract

BackgroundThe C-terminal four amino acids (GEEV) of human α1A-adrenergic receptors (ARs) have been reported to interact with the PDZ domain of neuronal nitric oxide synthase (nNOS) in a yeast two-hybrid system. The other two α1-AR subtypes have no sequence homology in this region, raising the possibility of subtype-specific protein-protein interactions.ResultsWe used co-immunoprecipitation and functional approaches with epitope-tagged α1-ARs to examine this interaction and the importance of the C-terminal tail. Following co-transfection of HEK-293 cells with hexahistidine/Flag (HF)-tagged α1A-ARs and nNOS, membranes were solubilized and immunoprecipitated with anti-FLAG affinity resin or anti-nNOS antibodies. Immunoprecipitation of HFα1A-ARs resulted in co-immunoprecipitation of nNOS and vice versa, confirming that these proteins interact. However, nNOS also co-immunoprecipitated with HFα1B- and HFα1D-ARs, suggesting that the interaction is not specific to the α1A subtype. In addition, nNOS co-immunoprecipitated with each of the three HFα1-AR subtypes which had been C-terminally truncated, suggesting that this interaction does not require the C-tails; and with Flag-tagged β1- and β2-ARs. Treatment of PC12 cells expressing HFα1A-ARs with an inhibitor of nitric oxide formation did not alter norepinephrine-mediated activation of mitogen activated protein kinases, suggesting nNOS is not involved in this response.ConclusionsThese results show that nNOS does interact with full-length α1A-ARs, but that this interaction is not subtype-specific and does not require the C-terminal tail, raising questions about its functional significance.