Abstract
The interaction of acridine orange and sodium poly-α, L-glutamate in the pH range of 3.8–6.8 was studied by a spectral method and viscosity measurement. From the binding data, the molar change in entropy, enthalpy and the number of binding sites on polymer were calculated. The values of ΔS° were positive. ΔS° for binding decreasing extensively in pH range of 5.0. These results are explained by the transition of helix ⇋ coil of the polymer. The intrinsic viscosity reduction in the presence of the dye, in pH 3.8 is due to the formation of dye bridges between the polymer helix.
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