Abstract

This study was conducted to evaluate the eiect of dietary levels of vitamins C (0, 100 and 2000 mg kg � 1 ), E (0, 50 and 500 mg kg � 1 ) and their interaction on the growth performance, liver contents of ascorbic acid and a-tocopherol, haematology and immune response of channel cat¢sh, Ictalurus punctatus. Each diet was fed to cat¢sh in triplicate aquaria to apparent satiation twice daily for 12 weeks. The results indicate that the amount of vitamin E contained in the basal diet (23.1mg kg � 1 )w as su⁄cient to promote good growth, feed e⁄ciency and survival, but its supplementation was needed to maintain high haematological values and liver vitamin E. Supplementation of vitamin C (100 mg kg � 1 ) to the basal diet containing 10.5 mg kg � 1 was required for good growth, feed e⁄ciency, survival and prevention of vertebral deformity and optimum haematological indices. Liver storage of ascorbic acid and a-tocopherol increased with increasing dietary levels of each vitamin. Dietary vitamin E levels had no eiect on liver ascorbic acid content, but increasing dietary vitamin C increased liver a-tocopherol. Some measured immune parameters (serum protein and superoxide anion production) were enhanced by supplementation of vitamin C or E. Chemotaxis ratio and phagocytosis were not aiected by treatments.

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