Abstract
AbstractIntegrin-mediated signal transduction and membrane blebbing have been well studied to modulate cell adhesion, spreading and migration^1-6^. However, the relationship between membrane blebbing and integrin signaling has not been explored. Here we show that integrin-ligand interaction induces membrane blebbing and membrane permeability change. We found that sodium-proton exchanger 1 (NHE1) and sodium-calcium exchanger 1 (NCX1) are located in the membrane blebbing sites and inhibition of NHE1 disrupts membrane blebbing and decreases membrane permeability change. However, inhibition of NCX1 enhances cell blebbing to cause cell swelling which is correlated with an intracellular sodium accumulation induced by NHE17. These data suggest that sodium influx induced by NHE1 is a driving force for membrane blebbing growth, while sodium efflux induced by NCX1 in a reverse mode causes membrane blebbing retraction. Together, these data reveal a novel function of NHE1 and NCX1 in membrane permeability change and blebbing and provide the link for integrin signaling and membrane blebbing.
Highlights
Yung-Hsiang Yi1,2, Yu-Sun Chang[1], Chi-Hung Lin[3], Tien-Shen Lew[4], Chih-Yung Tang[5], Tsung-Yu Chen[6], Wei-Lien Tseng[7], Ching-Ping Tseng[7] and Szecheng J
We found that sodium-proton exchanger 1 (NHE1) and sodium-calcium exchanger 1 (NCX1) are located in the membrane blebbing sites and inhibition of NHE1 disrupts membrane blebbing and decreases membrane permeability change
Integrin receptors, constituting 24 αβ heterodimers that result from combinations between 18 α and 8 β subunits, and their extracellular matrix (ECM) ligands i.e., collagen, laminin and fibronectin etc., regulate many of these cell activities[1,2,3], it is unclear whether integrin-mediated signals are involved in membrane blebbing that can cause the cells to exhibit cell adhesion and spreading
Summary
Yung-Hsiang Yi1,2, Yu-Sun Chang[1], Chi-Hung Lin[3], Tien-Shen Lew[4], Chih-Yung Tang[5], Tsung-Yu Chen[6], Wei-Lien Tseng[7], Ching-Ping Tseng[7] and Szecheng J. Membrane blebbing after CHOαIIbβ[3] cells attached onto the Fg-coated substrate was observed before cell spreading (Fig. 2a upper panels and supplemental Movie 1). CHOαIIbβ[3] cells plated onto the control bovine fetal albumin (BSA)-coated substrate did not show noticeable blebbing (Fig. 2a, lower panels and supplemental Movie 2).
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