Abstract
The feasibility of producing polyhydroxyalkanoate (PHA) from pretreated waste wood hydrolysate and volatile fatty acids (VFAs) from sewage fermented products as co-substrate feedstock through mixed microbial cultures (MMCs) process was explored. The results showed that the addition of co-substrate shortened the cycle of PHA reaching the maximum and increased the proportion of 3-hydroxyvalerate (3HV) monomer. Compared with N-excess supply, almost 1.6 times increased PHA accumulation was realized under N-limitation, and simultaneously the highest proportion of 3HV monomer with 51% was also obtained. Additionally, PHA production in S1400 reactor reached a maximum value of about 3088 mg COD/L with culture time to 36 h. The microbial community also displayed a high diversity, which was composed of 65 bacterial genera. It is a novel attempt to accumulate PHA from pretreated waste wood hydrolysate and VFAs co-substrate through MMCs, providing an effective green approach to reduce its expensive cost and achieve mass production.
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