Inside Cover: Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells (Angew. Chem. Int. Ed. 34/2023)

Abstract

The β-barrel assembly machine (BAM) is essential for folding outer membrane proteins (OMPs) into the outer membrane (OM) of Gram-negative bacteria. Structures of BAM have been solved using X-ray crystallography and cryoEM, but the conformation of BAM in the highly crowded native OM remained unsolved. Using EPR spectroscopy of BAM in intact E. coli cells, the structure of BAM in situ has now been revealed in the Research Article (e202218783) by Sheena E. Radford, Christos Pliotas et al., both alone and in the presence of the antibacterial, darobactin B. Image: Jeroen Claus (Phospho Biomedical Animation).