Abstract

Abstract Adult fleas were maintained on laboratory cats (4) housed in individual metal cages with metal mesh floors. The cages were tapered below the mesh and were fitted with a 1 -gal glass Jar into which flea eggs dropped from each cat and were collected. Larvae were reared in 1-qt jars on a pulverized mixture of dry dog chow, dried beef blood, and brewers yeast (10:1.5:1) mixed with equal parts of fine grain sand and sawdust. After 7 days, pupae were separated daily from the larval medium with a No. 16 screen. A glass funnel fitted with 16 mesh screen fastened to a Jar lid was used to separate adults and pupae. Adult fleas 2-5 days old were exposed to treated 78 cm2 plush nylon carpet discs. Liquid insecticides were applied with a B&G compression sprayer equipped with a Sprayer Systems multijet nozzle set to deliver a fine mist from 1-2 ft. Aerosol was sprayed to visible dampness. The Al applied was determined by immediately weighing filter paper sprayed simultaneously with the carpet (ca 0.10 mg/cm2 per 1% spray). Each treated disc was allowed to dry for ca 24 hr. Discs were placed, treated surface up, at the bottom of leucite cylinders (10.0 cm i.d. x 38.1 cm). Replicates (n = 3) of 30-120 fleas were placed onto the treated carpet discs and allowed to remain on the discs 4 hours. The discs were subsequently removed from the cylinder with long forceps and placed on a pedestal in a covered enamel pan containing ca 2 cm of water. Live fleas jumped off the carpet into the water and were counted. Dead fleas on the carpet were counted. Control mortality was corrected by Abbott’s Formula.

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