Innovative eco-friendly approaches to fresh lamb preservation with postbiotics from Lactobacillus acidophilus VBFDP771 and Shirazi balangu mucilage

Antimicrobial efficacy of drug blended biosynthesized colloidal gold nanoparticles from Justicia glauca against oral pathogens: A nanoantibiotic approach

A facile ecofriendly biological approach for the synthesis of selenium nanoparticles using the probiotic Lactobacillus acidophilus bacteria and its characterization is reported in this paper. The synthesized selenium nanoparticles were characterized using UV spectrometer, X-ray diffraction and transmission electron microscopy. The results of X-ray diffraction and transmission electron microscopic studies showed that the nanoparticles were in the uniform size range of 15-50 nm. The present study showed that no chemical changes occurred in selenium nanoparticles during the wet sterilization process and therefore, the wet sterilization method can effectively use to recover the elemental selenium from bacterial cells.

Effect on the cholesterol content of fresh lamb of supplementing the feed of Awassi ewes and lambs with Lactobacillus acidophilus

Chemotherapy side effects, drug resistance, and tumor metastasis hinder the progress of cancer treatments, which has led to cancer patients having a poor prognosis. In recent years, nanoparticles (NPs) have become an emerging trend in drug delivery technology. The biosynthesis of gold nanoparticles (AuNPs) from lactic acid as probiotic metabolites is a cost-efficient and eco-friendly approach due to the widespread availability and ease of culturing methods. Here, AuNPs were synthesized by reducing chloroauric acid using lactic acid isolated from the probiotic Lactobacillus acidophilus. Surface characterization of the biosynthesized gold nanoparticles was performed by UV-visible spectroscopy, field emission scanning electron microscopy with energy dispersive spectroscopy (FESEM-EDS), and atomic force microscopy (AFM). The distinct peak of the AuNPs in the UV-Vis spectra at around 520 nm indicates the nanoscale level of the gold particles. SEM images of the shape of the biosynthesized AuNPs were found to be small, smooth spherical particles with sizes ranging from 6 to 12 nm. AFM results agree with SEM images, showing AuNPs with sizes ranging from 4 to 15 nm. The anticancer activities of the biosynthesized AuNPs have been investigated by cytotoxicity (MTT) and apoptosis (Caspase 3/7) assay in the human breast and lung cancer cells (MCF7 and A549), and control (myoblasts). MTT assay showed AuNPs’ cytotoxic potential on cancer cells (MCF7 and A549) compared to normal cells (myoblasts). Cytotoxicity and apoptosis assay of the synthesized AuNPs exhibit toxicity against human breast adenocarcinoma cells (MCF7, IC50 of 0.075 mM) and human lung cancer cells (A549, IC50 of 0.07 mM), as shown evidently in the cellular morphology and in the DNA-stained nucleus. Only MCF7 cells exhibited apoptotic events upon AuNP treatment. AuNPs proved to be safe as they are non-toxic against normal cells and myoblasts. Interestingly, the biosynthesized AuNPs were absorbed by the cells and are present in the cytosol, thus demonstrating selectivity toward breast and lung cancer cells used. The study showed the first evidence that AuNPs can be synthesized using lactic acid as a reducing agent and capping agent isolated from the probiotic Lactobacillus acidophilus. The results suggested potential cancer chemotherapeutic leads and targeted delivery in human breast and lung cancers.

Bacteriocins from lactic acid bacteria (LAB) are useful to control the persistent development of pathogenic microorganisms in food and medicine fields. The bacteriocin acidocin was extracted from Lactobacillus acidophilus M1 that was isolated from fermented milk, purified using ammonium sulphate fractionation, and gel filtration column chromatography using Sephadex matrix and applied as a potential antibacterial agent. The molecular weight of the purified acidocin was estimated using SDS-PAGE to be 6.6 kDa. The acidocin was compared with silver nanoparticles (SNPs), biosynthesized by Aspergillus brasiliensis (niger) ATCC 16404, against two bacterial strains Bacillus cereus ATCC 14579 and Staphylococcus aureus ATCC 25923. Both acidocin and SNPs showed significance antibacterial effects using disc and well diffusion methods; the maximum antibacterial activity was proved against B. cereus from acidocin/SNPs composite using a ratio of 1/1 from each agent. The application of acidocin/SNPs composite as immersion solution, for disinfecting chicken eggshells, resulted in remarkable reduction in microbial load on the shells of 5.53 log10 CFU/eggshell. Results could provide an eco-friendly approach for potential antimicrobial composites to be used in food preservation and other health protection researches.