Abstract

We studied immunocytochemical localization of thyrotropin-releasing hormone (TRH) in the bullfrog pars intermedia. Many TRH-like immunoreactive terminals containing immunoreactive granular vesicles 80–150 nm in diameter (116 nm mean diameter) were found in the pars intermedia. Some TRH-like immunoreactive terminals were in synaptic contact with the intermedia cells. These findings support the hypothesis that TRH directly activates the intermedia cells via synapses to secrete α-melanocyte-stimulating hormone.

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