Abstract
The development of perspective diagnostic techniques in medicine requires efficient high-throughput biological sample analysis methods. Here, we present an inline cartridge extraction that facilitates the screening rate of mass spectrometry shotgun lipidomic analysis of tissue samples. We illustrate the method by its application to tumor tissue identification in neurosurgery. In perspective, this high-performance method provides new possibilities for the investigation of cancer pathogenesis and metabolic disorders.
Highlights
The development of perspective diagnostic techniques in medicine requires efficient high-throughput biological sample analysis methods
Prominent changes in energy metabolic pathways of malignant cells lead to intensive aerobic glycolysis[13] (Warburg effect) required to generate enough ATP14 and to the activation of de novo lipogenesis[15], which is proposed as a crucial step in cell proliferation[16]
Regarding brain tumors and neurosurgery, the most important methods are based on ambient mass spectrometry, or so-called direct tissue analysis, especially on desorption electrospray ionization[11,25], rapid evaporative ionization[29] or microextraction followed by electrospray ionization[30,31,32]
Summary
The development of perspective diagnostic techniques in medicine requires efficient high-throughput biological sample analysis methods. Www.nature.com/scientificreports preparation or a high amount of the tissue for analysis, so a simple, rapid and reliable method for direct tissue analysis is still required to transfer mass spectrometry profiling approaches to a clinic.
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