Abstract
The Melanin synthesis is controlled by a series of enzymatic reactions regulated at the level of tyrosinase in melanocytes and melanoma cells. In an ongoing search for new tyrosinase inhibitors from natural sources, the dried leaves of Schisandra chinensis were extracted with 70% acetone and separated into fractions 1-3 (Fr.1-Fr.3). All fractions were evaluated for their inhibitory activity potency against the tyrosinase, and Fr.2 showed potent tyrosinase activities with 34.7% inhibition concentration 1,000 μg/mL compared to the positive control, albutin (23.5%). Finally, melanin contents was measured to determine the whitening effect. Fr.2 was inhibited at 19.3% at 50μg/mL, respectively. We investigated the changes in protein level of tyrosinase, Melanocyte Inducing Transcription Factor (MITF) by using western blotting. Fr.2 was able to significantly inhibit tyrosinase expression in protein level. These results suggest that Fr.2 inhibited melanin production which may be dependent on tyrosinase activity and expression in B16F10 melanoma cells, and an effective whitening agent for the skin.
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