Inhibitory effects of Herceptin combined with mitomycin C on human bladder urothelial carcinoma T24 lines

Abstract

Objective To explore the anti-tumor effects of Herceptin and mitomycin C (MMC) on human bladder urothelial carcinoma T24 lines. Methods The SP immunohistochemical method and RT-PCR method were used to detect the expression of HER-2/neu in human bladder urothelial carcinoma T24 lines. MTT method was applied to assay the growth inhibition rate of various concentrations of Hercep-tin (10,20,40,80,and 160 μg/L),MMC (4,8,16,32,and 64 μg/L),Herceptin combined with MMC on human bladder urothelial carcinoma T24 lines. Results There was high expression of HER-2/neu in hu-man bladder urothelial carcinoma T24 lines by immunocytochemistry and RT-PCR. Herceptin slightly in-hibited proliferation of human bladder urothelial carcinoma T24 lines at the 72nd h as compared with that at the 48th h, all P<0.05). The combined use of herceptine and MCC exerted the synergistic effects at the 24th,48th,and 72nd h (q value: 1. 264 to 3. 473),and an additive effect was obtained at the 96th h (q value:0.913 to 1. 138). Conclusion Her-2/neu was overexpressed in human bladder urothelial carcino-ma T24 lines. Herceptin slightly inhibited proliferation of T24 lines and exerted the effects slowly. There was a synergistic effect to inhibit proliferation of T24 lines by combined use of herceptine and MMC. Key words: Bladder carcinoma; Herceptin; Gene expression; Mitomycin