Inhibitory Effect of Portulaca oleracea L. Polysaccharide on Porcine Rotavirus

Abstract

Porcine rotavirus (PoRV) infection can cause diarrhea, dehydration, vomiting and even death of piglets, which has caused great economic losses to the global pig industry. In this study, the inhibitory effect of Portulaca oleracea L. polysaccharide (POL-P) on porcine rotavirus (PoRV) was investigated by using porcine small intestine epithelial cells (IPEC-J2. CCK-8 method was used to detect the cytotoxicity of POL-P to IPEC-J2 cells. PoRV was infected with IPEC- J2, and a virus infection model was established in vitro. The maximum safe concentration of POL-P and the effect of POL-P on VP6 protein and mRNA expression were detected by CCK-8, Western blot and RT-qPCR. In addition, immunofluorescence was also used to detect the inhibition of VP6 protein expression of PoRV. The results showed that the cells treated with 100, 200, 400 μg/ml POL-P for 24 h had no toxic effect on the cells. POL-P showed inhibitory effects of protein and mRNA expression on the virus, and the concentration of 400 μg/ml displayed the best inhibitory activity. Indirect immunofluorescence detection showed that the VP6 protein expression was inhibited under POL-P treatment. In conclusion, POL-P has an obvious inhibitory effect on PoRV in vitro.