Inhibitory effect of DCPIB on proliferation of mouse microglia cell line BV-2

Abstract

Objective To study the inhibitory effect of DCPIB on mouse microglia cell line BV-2 and the mechanisms underlying such effect.Methods The microglia cell line BV-2 was incubated with DCPIB in different concentrations ( 10,20 and 40 μ mol/L) for different intervals (24,48 and 72 h).Cell inhibition ratio was assessed by methyl thiazol tetrazolium (MTT) assay and flow cytometry analysis was performed to detect the cell cycle.Furthermore,Cyclin D1 expression was detected by using Western blotting.Results MTT assay revealed that BV-2 cell growth curve moved down in a concentration- and timedependent manner.Cell grown was inhibited by DCPIB obviously,and the inhibition ratio was (29.20 ±3.99)％,(38.93 ±2.36)％,(68.52 ±2.16)％ at 48 h after treatment with DCPIB (10,20 and 40μmol/L).Flow cytometry showed that most BV-2 cells were inhibited by DCPIB and arrested in G0/G1 phase,while proportion of BV-2 cells in G2/M phase and S phase was decreased.Western blotting demonstrated that the expression level of Cyclin D1 was down-regulated in BV-2 cells after treatment with DCPIB for 72 h.Conclusion The results suggest that DCPIB can markedly inhibit the proliferation of BV-2 cells,which may be related with arrest of the cell cycle in G0/G1 phase and down-regulation of Cyclin D1 expression. Key words: Microglia; Volume regulated anion channels; Proliferation; Cell cycle