Abstract
The effect of 0.11 M sodium bicarbonate on trichothecene mycotoxin formation was determined by analyzing cultures of Fusarium tricinctum NRRL 13426 incubated in peptone- and saccharose-supplemented Czapek-Dox broth in the presence and absence of the base. Sodium bicarbonate-treated cultures exhibited a 7-51-fold decrease in the production of diacetoxyscirpenol (DAS) and T-2 toxin. The production of 15-acetoxyscirpenol, acetyl T-2 toxin, and neosolaniol was completely inhibited by the sodium bicarbonate. The analysis method (which could also detect 3-acetyl-DAS, deoxynivalenol, HT-2 toxin, scirpentriol, T-2 tetrol, T-2 tetraacetate, and T-2 triol) employed cold on-column capillary gas chromatography/isobutane chemical ionization ion trap mass spectrometry of the underivatized mycotoxins.
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