Abstract
Rabbit platelet cyclic AMP phosphodiesterase is inhibited by the three calcium channel blockers nifedipine, diltiazem, and verapamil with IC 50's of 100 μM, 100 μM and 420 μM, respectively. Also, platelet aggregation induced by 4 μM ADP is inhibited by those compounds. Verapamil is the most potent aggregation inhibitor with an IC 50 of 260 μM while diltiazem and nifedipine have IC 50's of 630 μM and 840 μM, respectively. All three compounds display a maximum inhibition of 80–85%. Diltiazem and PGD 2potentiate the antiaggregatory activity of each other in that the inhibitions occurring in the presence of the combination of the two (at varying concentrations) exceed the calculated sums of the inhibitions produced by each alone. On the other hand, the antiaggregatory activities of verapamil or nifedipine, are additive with that of PGD 2 in that no significant differences exist between the observed inhibitions produced by the combinations and the calculated summed values of the individual inhibitions. Our data suggest, therefore, that in addition to lowering intracellular calcium ions, which are required for platelet aggregation, the three calcium channel blockers inhibit the breakdown of cyclic AMP thereby promoting antiaggregation.
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