Abstract
The effect of trachyloban-19-oic acid isolated from Iostephane heterophylla (Asteraceae), was investigated on several photosynthetic activities in spinach thylakoids. The results indicated that this compound inhibited ATP synthesis and uncoupled electron transport, as well as basal and phosphorylating electron flow. Therefore, trachyloban-19-oic acid acts as Hill reaction inhibitor. This compound did not affect photosystem I activity but inhibited uncoupled photosystem II electron flow from H 2O to DCPIP, and has not effect on electron flow from H 2O to SiMo, indicating that the site of inhibition of this compound is at the level of Q A–Q B. Chlorophyll a fluorescence measurements confirm the behavior of this diterpene as Q A–Q B inhibitor, and in the other hand, this results indicate that a perturbation in the thylakoid membranes at the level of LHC II occurs.
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